HomeSingle particle cryoEM at Cornell UniversityInstrument access and training

Instrument access and training

It is recommended you approach John or Mariena for cryoEM related inquiries. Typically we’ll meet one-on-one to discuss your goals, sample(s), and timeline. John is available for training and consulting at Duffield Hall, while Mariena is available in Physical Sciences. Once the goals are established, new users should request an account on Coral. Coral serves as the main hub for booking time and tracking usage of the microscope. Users should coordinate with facility managers for individual training. The overall goal is for users to learn how to prepare (stain and freeze) samples and perform data collection themselves. To this end,  hours of training should be completed at Duffield Hall and/or 50 hours should be completed at Physical Sciences. Typical workflow is as follows:

  1. Meet one-on-one with managers to discuss project(s), timeline and goals.
  2. Request access for Coral.
  3. Perform negative stain experiment(s) for initial visualization and sample characterization.
  4. Optimize amount of sample, dispersity, quality and determine feasibility of the project.
  5. Complete training on the Spirit or BioTwin (negative stain and initial cryo experiments).
  6. Sample optimization for cryo conditions on the Tecnai F20.
  7. Determine if what voltage(s) is (are) needed for desired results.
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